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KMID : 1094720090140020180
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Biotechnology and Bioprocess Engineering
2009 Volume.14 No. 2 p.180 ~ p.186
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Comparison of the Synthetic Biodegrad-able Polymers, Polylactide (PLA), and Poly-lactic-co-glycolic acid (PLGA) as Scaffolds for Artificial Cartilage
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Lee Nam-Kyoung
Oh Ho-Jung
Hong Choong-Man
Suh Hwal
Hong Seung-Hwa
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Abstract
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Chondrocytes are easily de-differentiated when cultured in monolayer, and tissue-engineered cartilage can be generated by seeding chondrocytes onto three-dimensional porous synthetic biodegradable polymers. In this study, we investigated the biochemical and molecular aspects of chondrocytes in a monolayer-culture system and selected the optimal subculture passages based on their de-differentiation. We also compared two commonly used synthetic biodegradable polymers, polylactide (PLA), and polylactic-co-glycolic acid (PLGA), for their suitability as scaffolds for artificial cartilage. De-differentiated chondrocytes were observed after two passages. These results suggested that the first cell passage was optimal for seeding as only a few chondrocytes secreted extracellular matrix components to form homogeneously compact cartilage. Sub-stantially increased glycosaminoglycan and total collagen levels revealed that PLGA scaffolds were a better option for inducing cartilage tissue formation compared to the PLA scaffolds. Histological and immunohistochemical results showed that chondrocytes seeded into PLGA retained their morphological pheno-type to a greater extent than those seeded into PLA.
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KEYWORD
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artificial cartilage, biodegradation, polymer, chondrocyte, biocompatibility, biological safety
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